The purpose of this study was to analyze the effect of emotional intelligence of skin care workers on self-efficacy and job stress (internal stress, external stress), and to verify whether self-efficacy has a mediating effect in the relationship between these variables. To this end, a survey was conducted on 195 heads of skin care offices living across the country, and multiple regression analysis and mediating effect analysis were performed on the collected data. As a result of the study, first, it was found that understanding self-emotion, emotion regulation, and understanding of other people’s emotions, which are sub-factors of emotional intelligence, all had a significant positive (+) effect on self-efficacy. Second, emotional intelligence had a significant negative (-) effect on internal and external stress, and in detail, it was confirmed that understanding self-emotion and understanding of other people’s emotions had a significant effect on internal stress, and understanding self-emotion and emotion regulation had a significant negative (-) effect on both internal and external stress. Fourth, self-efficacy played a significant role as a parameter in the relationship between emotional intelligence and job stress, and partial and complete mediating effects were varied for each sub-factor. These results suggest that the improvement of emotional intelligence and self-efficacy of skin care workers can contribute to reducing job stress, and emphasize the need for educational programs to strengthen emotional management and self-efficacy.